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anti b7 h3 antibody  (R&D Systems)


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    Structured Review

    R&D Systems anti b7 h3 antibody
    A. Immunocytochemistry (ICC) analysis demonstrates strong B7-H3 (CD276) expression on both PC-3 cancer cells and pCAFs, visualized by red fluorescence. B. In 2D co-culture assays, <t>anti-B7-H3</t> CAR T cells exhibit antigen-specific cytotoxicity against PC-3 cells, with killing efficiency increasing across graded effector-to-target (E:T) ratios.
    Anti B7 H3 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b7+h3/bio_rxiv__64898__2026__04__15__718753-197-2-5?v=R%26D+Systems
    Average 93 stars, based on 88 article reviews
    anti b7 h3 antibody - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Transcription Factor–Mediated Reprogramming of Cancer-Associated Fibroblasts Reveals Targetable Vulnerabilities in Solid Tumors"

    Article Title: Transcription Factor–Mediated Reprogramming of Cancer-Associated Fibroblasts Reveals Targetable Vulnerabilities in Solid Tumors

    Journal: bioRxiv

    doi: 10.64898/2026.04.15.718753

    A. Immunocytochemistry (ICC) analysis demonstrates strong B7-H3 (CD276) expression on both PC-3 cancer cells and pCAFs, visualized by red fluorescence. B. In 2D co-culture assays, anti-B7-H3 CAR T cells exhibit antigen-specific cytotoxicity against PC-3 cells, with killing efficiency increasing across graded effector-to-target (E:T) ratios.
    Figure Legend Snippet: A. Immunocytochemistry (ICC) analysis demonstrates strong B7-H3 (CD276) expression on both PC-3 cancer cells and pCAFs, visualized by red fluorescence. B. In 2D co-culture assays, anti-B7-H3 CAR T cells exhibit antigen-specific cytotoxicity against PC-3 cells, with killing efficiency increasing across graded effector-to-target (E:T) ratios.

    Techniques Used: Immunocytochemistry, Expressing, Fluorescence, Co-Culture Assay

    Stromal Effects on CAR T-Cell Activity in 3D-Bioprinted PC-3 Spheroids. A. Representative images of stromal populations influencing anti-B7-H3 CAR T-cell killing function . B. PC-3 spheroid with pCAF/rpNFs interaction with CAR-T cells monitored by IncuCyte imaging system. C. The killing effects of B7-H3 CAR T cells on PC-3 cells in day 5 compared to day 1. D . Imaging of live cells at different time.
    Figure Legend Snippet: Stromal Effects on CAR T-Cell Activity in 3D-Bioprinted PC-3 Spheroids. A. Representative images of stromal populations influencing anti-B7-H3 CAR T-cell killing function . B. PC-3 spheroid with pCAF/rpNFs interaction with CAR-T cells monitored by IncuCyte imaging system. C. The killing effects of B7-H3 CAR T cells on PC-3 cells in day 5 compared to day 1. D . Imaging of live cells at different time.

    Techniques Used: Activity Assay, Imaging



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    anti b7 h3 antibody  (R&D Systems)
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    A. Immunocytochemistry (ICC) analysis demonstrates strong B7-H3 (CD276) expression on both PC-3 cancer cells and pCAFs, visualized by red fluorescence. B. In 2D co-culture assays, <t>anti-B7-H3</t> CAR T cells exhibit antigen-specific cytotoxicity against PC-3 cells, with killing efficiency increasing across graded effector-to-target (E:T) ratios.
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    A. Immunocytochemistry (ICC) analysis demonstrates strong B7-H3 (CD276) expression on both PC-3 cancer cells and pCAFs, visualized by red fluorescence. B. In 2D co-culture assays, <t>anti-B7-H3</t> CAR T cells exhibit antigen-specific cytotoxicity against PC-3 cells, with killing efficiency increasing across graded effector-to-target (E:T) ratios.
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    Image Search Results


    A. Immunocytochemistry (ICC) analysis demonstrates strong B7-H3 (CD276) expression on both PC-3 cancer cells and pCAFs, visualized by red fluorescence. B. In 2D co-culture assays, anti-B7-H3 CAR T cells exhibit antigen-specific cytotoxicity against PC-3 cells, with killing efficiency increasing across graded effector-to-target (E:T) ratios.

    Journal: bioRxiv

    Article Title: Transcription Factor–Mediated Reprogramming of Cancer-Associated Fibroblasts Reveals Targetable Vulnerabilities in Solid Tumors

    doi: 10.64898/2026.04.15.718753

    Figure Lengend Snippet: A. Immunocytochemistry (ICC) analysis demonstrates strong B7-H3 (CD276) expression on both PC-3 cancer cells and pCAFs, visualized by red fluorescence. B. In 2D co-culture assays, anti-B7-H3 CAR T cells exhibit antigen-specific cytotoxicity against PC-3 cells, with killing efficiency increasing across graded effector-to-target (E:T) ratios.

    Article Snippet: Primary human anti–B7-H3 antibody (AF1027, R&D Systems) was diluted in cold staining buffer (0.5 μg/μL; 2 μL per 100 μL reaction volume) and applied to cells for 20–30 min at 4 °C.

    Techniques: Immunocytochemistry, Expressing, Fluorescence, Co-Culture Assay

    Stromal Effects on CAR T-Cell Activity in 3D-Bioprinted PC-3 Spheroids. A. Representative images of stromal populations influencing anti-B7-H3 CAR T-cell killing function . B. PC-3 spheroid with pCAF/rpNFs interaction with CAR-T cells monitored by IncuCyte imaging system. C. The killing effects of B7-H3 CAR T cells on PC-3 cells in day 5 compared to day 1. D . Imaging of live cells at different time.

    Journal: bioRxiv

    Article Title: Transcription Factor–Mediated Reprogramming of Cancer-Associated Fibroblasts Reveals Targetable Vulnerabilities in Solid Tumors

    doi: 10.64898/2026.04.15.718753

    Figure Lengend Snippet: Stromal Effects on CAR T-Cell Activity in 3D-Bioprinted PC-3 Spheroids. A. Representative images of stromal populations influencing anti-B7-H3 CAR T-cell killing function . B. PC-3 spheroid with pCAF/rpNFs interaction with CAR-T cells monitored by IncuCyte imaging system. C. The killing effects of B7-H3 CAR T cells on PC-3 cells in day 5 compared to day 1. D . Imaging of live cells at different time.

    Article Snippet: Primary human anti–B7-H3 antibody (AF1027, R&D Systems) was diluted in cold staining buffer (0.5 μg/μL; 2 μL per 100 μL reaction volume) and applied to cells for 20–30 min at 4 °C.

    Techniques: Activity Assay, Imaging